杨恒林¹  杨品芳¹  刘德全²  刘瑞君²  董莹¹  张春勇²  高大庆²  何 慧¹

1．云南省疟疾防治研究所    云南思茅 665000

2．中国预防医学科学院寄生虫病研究所    上海  200025

全文发表于《中国寄生虫学与寄生虫病杂志》1992年第10卷第3期198-200页

 

提要：1988年及1990年在滇南恶性疟流行区用体外微量法测得恶性疟原虫对氯喹、咯萘啶、青蒿琥脂及哌喹的抗性率分别为98.7％（75／76）、  27.6%（16／58）、13.8％（9／65）及97.75（43／44）；半数抑制量（ID₅₀）分别为125.0、19.0、4.7及243.3nmol／L，抗氯喹恶性疟原虫对哌喹有明显的交叉抗性；对咯萘啶及青蒿琥酯则未见明显的交叉抗性。抗青蒿琥酯恶性疟原虫对以上3种药均有一定程度的交叉抗性。抗咯萘啶恶性疟原虫对青蒿琥酯无交叉抗性，而对氯喹及哌喹有一定程度的交叉抗性。

关键词：恶性疟原虫，体外测定，氯喹，咯萘啶，青蒿琥酯，哌喹

 

 

SENSITIVITY IN VITRO OF PLASMODIUM FALCIPARUM TO CHLOROQUINE, PYRONARIDINE, ARTESUNATE AND PIPERAQUINE IN SOUTH YUNNAN

Yang Heng-Lin, Yang Pin-Fang, Liu De-Quan, Liu-Rui-Jun

Dong Ying, Zhang Chun-Yong, Gao Da-Qing, He Hui

Institute of Malaria Prevention and Treatment of Yunnan Province, Simao 665000

Institute of Parasistic Diseases, Chinese Academy of Preventive Medicine, Shanghai 200025

 

ABSTRACT. The sensitivity of P.falciparum to chloroquine，pyronaidine，artesunate and piperaquine(CQ，PD，AT，PQ) was assayed using in vitro microtechnique in south Yunnan in 1990. The resistance rates were 98.7％（75／76），27.6（16／58），13.8％（9／65）and 97.7％(43 ／44) repectively, and ID₅₀ were 125.0, 19.0, 4.7 and 243.3nmol/L, respectively. The resistance rate against CQ showed no change as compared to the rates against CQ 5 and 9 years ago；but the ID₅₀ was lower. CQ-resistant P. falciparum showed a marked cross-resistance to Pq, but not to Pd and AT. AT-resistant P. falciparum exhibited cross-resistance to the above- mentioned three drugs. PD-resistant P. falciparum showed no cross redistance to AT, but showed cross resistance to CQ and PQ.

In comparison with chlroquine-coated plates, the plates coated with pyrongaridine, artesunate or piperaquine gave similar results as the former, which were shown by the rise in schizont inhibition rates along with the rise in drug concentration It indicates that pyrona-ridine-, artesunate-, and piperaquine-coated plates can be used in the assay of sensitivity of P. falcipanum to the three drugs.

Key words：Plasmodium falciparum, in vitro microtechnique, chloreoquine, pyronaridine, artesunate, piperaquine.

 

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