许建卫  顾云安  杜尊伟

云南省疟疾防治研究所  思茅  665000

全文发表于《医学动物防制》1994年第10卷第2期74-76页

 

摘要：本研究实验过程的观察和实验结果表明，致倦库蚊和三带喙蚊库蚊四龄幼虫虽然对多数杀虫剂产生抗生，但1龄幼虫和2龄初期幼虫敏感，其中致倦库蚊4龄幼虫景谷株对杀螟硫磷和元阳株对DDT的LC₅₀分别是一龄幼虫的65.2倍和34.9倍。最后文章分析了所得实验结果的原因，并对蚊虫抗药性在幼虫个体发育中的形成过程及机理进行了探讨。

关键词：敏感性，致倦库蚊，三带喙库蚊，1至4龄幼虫，杀虫剂

 

A STUDY ON THE SUSCEPTIBILITY OF INSTARⅠ TO Ⅳ LARVAE OF CULEX PIPIENS QUINQUEFASCIATUS AND C. TRITAENIORHNCHUS TO SEVEN INSECTICIDES

Xu Jianwei，Gu Yunan，Du Zunwei

Yunnan Institute of Malaria Prevention and Treatment，Simao   665000

 

Abstract.The observation of experimental process and the results on this study showed that instar Ⅳlarvaes of Culex pipiens quinquefasciatus and C. tritaeniorhynchus resistance to majority insecticides，but instar Ⅰ larvaes and initial stage of instar Ⅱ are susceptible to them. The LC₅₀ of instar Ⅳ of C. pipiens quinquefasciatus，jinggu strain to Fenitrothion and Yuanyang strain to DDT were respectively 65.2 times and 34.9 times of that of their instar Ⅰ. At last this paper analyses why the study gets these results，and explores the developed process and mechanism of mosquito resistance during individual development of larvae.

Key words:Susceptibility，Culex pipiens quinquefasciatus，Culex tritaeniorhynchus，instar Ⅰto Ⅳ larvae，insecticide

 

IN VITRO RESPONSE OF PLASMODIUM FALCIPARUM ON THE CHINA-VIETNAM BORDER TO NINE ANTIMALARIALS

Yang Heng-lin¹, Liu De-quan², Huang Kai-guo and Zhang Chun-yong²

1 Institute of Malaria Prevention and Treatment of Yunnan province, Simao, Yunnan665000;

   2 Institute of Parasitic Diseases, Chinese Academy of preventive Medicine, Shanghai200025,People’s Republic of China

Abstract：August-October,1993,fifty cases of slide-confirmed falciparum malaria found in the vicinity of Manhao town, which is situated at the southeastern part of Yunnan Province near the China-Vietnam border,were screend for in vitro Suscepti-

Bility to chloroquine, piperaquine,amodiaquine, artesunate, dihydro-artemisinine,

arteether,pyronaridine, mefloquine and quinine by use of the microtechnique. The resistance  rates of Plasmodium falciparum to the above mentioned nine antimalarials were 78.9％（30／38），72.9％(27/37)，85.3％（29／34），5.6％（2／36），13.3％（4／35），20.6％（7／34），22.2％（8／36），0（0／18），and 0（0／33），respectively. IC₅₀ （nmol/L）in the given order were 136, 228, 64, 4, 8,80, 24, 88 and 352, respectively, The mean concentrations to totally inhibit schizonts(nmol/L)were 452, 1,102,210, 24, 30, 360, 62,218 and 1, 482, respectively. The results indicate that the local strains of P. falciparum have developed widespread resistance to chloroquine, piperaquine and amodiaquine. In contast, a large proportion of the isolates were sensitive to artesunate, dihydroartemisinine,arteether and pyronaridine, while a few isolates showed low-grade resistance to these compounds. All the isolates tested were sensitive to mefloquine and quinine. Judging from these findings it is inadvisable to use 4-aminoquines in the treatment and prophylaxis of falciparum malaria in the locality.Under the condition of strengthened surveillance, artemisinine and its derivatives,pyronaridine,mefloquine as well as quinine can be used in the treatment of falciparum malaria in this area.

 

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